biological source
rat liver
packaging
tube of 5 μg 94121649-DNA-5UG, pkg of vial of cells 94121649-1VL
growth mode
Adherent
karyotype
Diploid or pseudodiploid
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Feeder cells for co-culture with freshly isolated hepatocytes.
Biochem/physiol Actions
Isolated by Dr Hayden Coon. Flat epithelial cell colonies that appear upon low density plating as suspensions of collagenase dissociated buffalo rat liver. Cell origin not clearly defined.
Rat epithelial cells
Preparation Note
Coon′s modified Ham′s F12 + 2mM Glutamine + 5% Foetal Bovine Serum (FBS) or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 5% Foetal Bovine Serum (FBS) + 2mM Glutamine.
Spit confluent cultures 1:100 i.e. seeding at 1x1,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.