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PNT2 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 95012613, human prostate, Epithelial

别名:

PNT-2 Cells

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关于此项目

UNSPSC Code:
41106514
Biological source:
human prostate
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Epithelial

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biological source

human prostate

packaging

tube of 5 μg 95012613-DNA-5UG, pkg of vial of cells 95012613-1VL

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

相关类别

Application

Biology of prostate cells, model of differentiated prostate epithelial cells.
PNT2 cell line has been used:
  • to assess the effects of Verapamil, an L-type calcium channel blocker on autophagy
  • to assess the cytotoxicity of aqueous dilutions of a universal self-priming dental adhesive (DA)
  • to study the expression of soluble adenylyl cyclase (sAC) in the cells

Biochem/physiol Actions

Established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from a prostate of a 33 year old male at post mortem. PNT2 cells contain the SV40 genome and express large T protein. They possess a well differentiated morphology with the expression of cytokeratin 8,18 and 19 with the latter being a feature of differentiated luminal cells of the glandular prostate. Cytokeratin 14, a marker of epithelial basal cells, is not expressed. The cells are non-tumourigenic in nude mice.
Human prostate normal, immortalised with SV40
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 8,13
D16S539: 9
D5S818: 12,13
D7S820: 11
THO1: 6,9.3
TPOX: 8,9
vWA: 17

Preparation Note

RPMI 1640 + 2mM Glutamine + 6 -10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

法规信息

常规特殊物品
低风险生物材料
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