HSG (HeLa derivative)

Human cervix carcinoma

The cell line HSG was originally thought to be established from a human submandibular gland from a 54 year old Japanese male. The cell line has since been found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.

MEM +2mM Glutamine +1% Non Essential Amino Acids (NEAA) + 10% New Born Calf Serum (NBCS).

Split confluent cultures 1:4 to 1:10 ie seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂, 37°C

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