biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 95040312-DNA-5UG, pkg of vial of cells 95040312-1VL
ImmunoDon
BALB/c mouse spleen
Myeloma
Sp2/0 Ag-14
Reactivity
Anti-Measles Virus Nucleoprotein
technique(s)
cell culture | mammalian: suitable
isotype
IgG2a
shipped in
dry ice
storage temp.
−196°C
Immunogen
Measles virus (strain Hallé)
Application
Diagnosis, study of measles virus infection
Biochem/physiol Actions
Mouse Sp2/0 x Mouse BALB/c spleen
NP cl.120 monoclonal antibody is one of a range of well characterised measles virus antibodies used to measure synthesis and topological accumulation of measles virus antigen during the lytic cycle. This anti-nucleoprotein antibody is negative for haemagglutinin and hemolysin inhibition as well as neutralisation assays and gives immunofluorescence only on fixed tissue.
Preparation Note
Maintain cultures between 1-5x100,000 cells/ml; 5% CO2; 37°C
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.