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UNSPSC Code:
41106514
Biological source:
human unknown/unspecified
Relevant disease(s):
cancer
Growth mode:
Suspension
Karyotype:
Near-tetraploid (8q+)
Morphology:
Lymphoblastoid
biological source
human unknown/unspecified
packaging
tube of 5 μg 95050415-DNA-5UG, pkg of vial of cells 95050415-1VL
growth mode
Suspension
karyotype
Near-tetraploid (8q+)
morphology
Lymphoblastoid
products
Immunoglobulin A (IgA)
receptors
Transferrin, CD38
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Chemotherapeutic agent studies, receptor studies, carcinogenesis assays.
Biochem/physiol Actions
Human Caucasian IgA-producing plasmacytoma
STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 12
D16S539: 9,13
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8,11
vWA: 14,15
CSF1PO: 11
D13S317: 12
D16S539: 9,13
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8,11
vWA: 14,15
This IgA-producing cell line has been established from a malignant effusion in a 62-year-old Caucasian woman with myeloma. They are positive for plasma cell antigen 1 (PCA-1), transferrin receptor and CD38 but negative for early B cell developmental markers. HLA DR and CALLA. NCI-H929 is negative for Epstein-Barr virus nuclear antigen. A rearrangement of the c-myc proto-oncogene has been observed as well as the expression of c-myc RNA and an activated ras allele.
Preparation Note
Maintain cultures between 2-4 x 100,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).
Other Notes
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法规信息
低风险生物材料
常规特殊物品
此项目有
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