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UNSPSC Code:
41106514
Biological source:
human uterus
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Modal no. 45, monosomic forms of chromosomes 5, 6 and 7
Morphology:
Epithelial
biological source
human uterus
packaging
tube of 5 μg 95051030-DNA-5UG, pkg of vial of cells 95051030-1VL
growth mode
Adherent
karyotype
Modal no. 45, monosomic forms of chromosomes 5, 6 and 7
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity and chemotherapeutic agent studies
Biochem/physiol Actions
A human uterine sarcoma derived in 1980 from a tumour obtained at the time of a hysterectomy from a 56 year old Caucasian female. The cells form tumours in athymic nude mice, readily form colonies in soft agar and have a reported doubling time of 22-24 hours. The cells are sensitive to a number of chemotherapeutic agents including doxorubicin, dactinomycin, mitomycin C, taxol and bleomycin. They are resistant to vinblastine dacarbazine, cisplatin, melphalan, vincristine methotrexate and etoposide. The multi drug-resistant cell line MES-SA/Dx5 was established from MES-SA cells grown in the presence of doxorubicin.
Human uterus sarcoma
STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11,13
D16S539: 11,12
D5S818: 13
D7S820: 7,11
THO1: 6
TPOX: 8,11
vWA: 18
CSF1PO: 11
D13S317: 11,13
D16S539: 11,12
D5S818: 13
D7S820: 7,11
THO1: 6
TPOX: 8,11
vWA: 18
Preparation Note
McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:6 to 1:8 i.e. seeding at 1-4 x 10,000 cells/cm2 using 0.03% EDTA; 5% CO2; 37°C. Do not use trypsin.
Other Notes
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法规信息
低风险生物材料
常规特殊物品
此项目有
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