My-La CD4+

Study of antigen-independent proliferation of T- cell lyphoma from mycosis fungoides patient.

Human Caucasian CD4+ T Lymphocyte, mycosis fungoides

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,12
D16S539: 8,11
D5S818: 9,12
D7S820: 10,11
THO1: 6
TPOX: 8,12
vWA: 17

Two different continuous cell lines were established from a plaque biopsy of an 82-year old male with mycosis fungoides stage II by inclusion of IL-2 and IL-4 in the culture medium: My-La, CD4+, (referred to in the literature as My-La, marker chromosome) and My-La, CD8+ (referred to in the literature as My-La 46,XY-18+i(18q) (Sigma Catalogue number. 95051033). My-La CD4+ is strictly dependent on exogenous IL-2 and IL-4 in the culture medium whereas My-La CD8+ on IL-2 only. Both show characteristic chromosome markers and are double homozygous for HLA-A1.B8. My-La CD8+ expresses β-13 of the T cell receptor and phenotypically weak staining was observed for CD2, CD3, CD8, CD25, CD71 and TCR-1 α / β. My-La CD4+ expresses β-18 of the T cell receptor plus staining for CD2, CD3, CD4,CD25, CD71, HLA-DR and TCR-1 α/ β. There has been no evidence of HTLV-I infection in the cell lines and serum from the patient did not demonstrate antibodies against HTLV-I and HTLV-II. Both cell lines show a low viability after resuscitation from frozen and a long lag time phase until exponential growth is reached.

RPMI 1640 (R0883) + 2mM L-Glutamine (G7513) + 10 U/ml IL-2 (H7041) + 10 U/ml IL-4 (H7291) + 10% Human AB Serum (H4522)

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37°C. Split only 1:2 as maximum dilution: freeze in 45% growth medium, 45% human AB serum plus 10% DMSO, low viability on resuscitation. Use of human serum produces high levels of debris in cultures. Population doubling time 20-24 hours.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.