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关于此项目
UNSPSC Code:
41106514
eCl@ss:
32160703
Biological source:
human lung (liver metastasis)
Relevant disease(s):
metastasis
Growth mode:
Semi-adherent aggregates
Karyotype:
Modal no. 41-42
Morphology:
Not specified
Manufacturer/tradename:
ECACC
产品名称
DMS 153, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 95062827, human lung (liver metastasis), Not specified
biological source
human lung (liver metastasis)
manufacturer/tradename
ECACC
packaging
tube of 5 μg 95062827-DNA-5UG, pkg of vial of cells 95062827-1VL
growth mode
Semi-adherent aggregates
karyotype
Modal no. 41-42
morphology
Not specified
products
Not specified
receptors
Bombesin, Epidermal growth factor (EGF)
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Application
Human lung tumour model
Biochem/physiol Actions
DMS 153 was isolated from the liver of a 44 year-old male patient at autopsy who had been treated with cytoxan and methotrexate. The cells were identified as small cell carcinoma of the lung (SCLC) and secrete moderate quantities of calcitonin (CT) and calcitonin gene related peptide (CGRP). Production of adrenocorticotropin (ACTH), bombesin (BN), 17 β-estradiol and oxytocin-neurophysin (OT-NP) has also been reported. The expression of cell surface antigens Leu-7, HLA-DR, My 23 (defined by AML-2-23 monoclonal antibody), the AML-1-99 defined antigen and CR3 receptor (defined by the OKM antibody) has been shown. DMS 153 cells produce receptors for BN and epidermal growth factor (EGF) and are tumourigenic in athymic, nude mice.
Human Caucasian lung small cell carcinoma, metastasis to liver
STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 10
D13S317: 13
D16S539: 12,13
D5S818: 12
D7S820: 8,10
THO1: 7,9.3
TPOX: 11
vWA: 14,15
Amelogenin: X,Y
CSF1PO: 10
D13S317: 13
D16S539: 12,13
D5S818: 12
D7S820: 8,10
THO1: 7,9.3
TPOX: 11
vWA: 14,15
Preparation Note
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Cells grow in large clumps.
Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Disclaimer
This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.