biological source
human lung (effusion)
packaging
tube of 5 μg 95062830-DNA-5UG, pkg of vial of cells 95062830-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Human lung tumour model
Biochem/physiol Actions
DMS 273 was initiated in 1978 from the pleural fluid specimen from a 50 year old female patient with small cell carcinoma of the lung. The patient was in relapse after chemotherapy and radiotherapy. DMS 273 cells exhibit intercellular processes and express retinoblastoma mRNA and protein. The cell line is tumorigenic in nude mice.
Human lung, small cell carcinoma
STR-PCR Data: Amelogenin: X
CSF1PO: 10 ,12
D13S317: 12,13
D16S539: 11
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 10
vWA: 15
CSF1PO: 10 ,12
D13S317: 12,13
D16S539: 11
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 10
vWA: 15
Preparation Note
Split sub-confluent cultures (70-80%) 1:4 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).
Other Notes
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法规信息
低风险生物材料
常规特殊物品
此项目有
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