biological source
mouse pancreas
packaging
tube of 5 μg 95062833-DNA-5UG, pkg of vial of cells 95062833-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Polymorph
products
Somatostatin and Insulin
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Study of exocrine pancreas carcinomas, hormone secretion studies.
Biochem/physiol Actions
Mouse pancreatic small cell anaplastic carcinoma
TGP 55 has been obtained from a small cell anaplastic carcinoma arising in the pancreas of a transgenic Tg(Ela-1SV40E)Bri 18 mouse these mice carry a pseudogene construct composed of a pancreatic-specific elastase-1 promoter linked to the coding region for SV40 T antigen and thereby represent an animal model for pancreatic carcinogenesis. TGP 55 cells are SV40T antigen positive and secrete somatostatin, it is one of a series of cell lines isolated from pancreatic tumours deposited at ECACC. Cells secrete somatostatin and insulin in culture. Adherent clumps of small cells.
Preparation Note
DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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法规信息
低风险生物材料
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相关内容
ECACC pancreatic cancer cell lines for various applications including drug response studies.
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