biological source
rabbit eye
packaging
tube of 5 μg 95081046-DNA-5UG, pkg of vial of cells 95081046-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Corneal epithelial research
Biochem/physiol Actions
Rabbit corneal epithelial cells, SV40 transformed
The RCE cell line, also known as K′RCE, was established after infection of primary cultured rabbit (New Zealand albino) corneal epithelial cells with a recombinant SV40 vector. The cells exhibit a cobblestone-like appearance and stratification, including desmosome formation and microvilli development. Expression of keratin AE1 was always detectable whereas AE5 was expressed only at confluency.
Preparation Note
DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 5μg/ml Insulin + 10ng/ml EGF + 0.1μg/ml Cholera toxin + 0.5% Dimethyl Sulphoxide (DMSO) + 15% Foetal Bovine Serum (FBS) (Heat Inactivated).
Split sub-confluent cultures (70-80%) 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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法规信息
低风险生物材料
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