生物来源
rat pancreas
包装
tube of 5 μg 95090402-DNA-5UG
pkg of vial of cells 95090402-1VL
生长模式
Adherent
核型
Not specified
形态学
Not specified
产品
Insulin, L-dopa-decarboxylase
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
细胞系来源
Rat islet cell tumour
细胞系描述
RIN-5F is a secondary clone of the rat islet tumour cell line RIN-m (Sigma Catalogue number. 95071701). Cloning was performed by limited dilution in RPMI 1640, 10%FBS and 20% conditioned medium. The conditioned medium contained growth hormones and prolactin being produced by rat pituitary tumour cells GH3 (Sigma Catalogue number. 87012603). RIN-5F cells secrete insulin, produce high levels of the key APUD enzyme, L-dopa-decarboxylase and contain membrane-bound secretory granules.
应用
Study of pancreatic islet cell biology eg. synthesis, storage and secretion of insulin
培养基
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells grow in small islands and do not form a confluent monolayer.
其他说明
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