biological source
human cervix
packaging
tube of 5 μg 95091410-DNA-5UG, pkg of vial of cells 95091410-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Gene activation and expression studies
Biochem/physiol Actions
Human cervix carcinoma, HeLa derivative, transformed
The HtTA-1 cell line was derived from HeLa cells being stably transfected with a tetracycline-controlled transactivator (tTA) consisting of the tet repressor fused with the activating domain of virion protein 16 of herpes simplex virus. This transactivator stimulates transcription from a minimal promoter sequence derived from the human cytomegalovirus promoter IE combined with tet operator sequences. This system allows differential control of the activity of an individual gene in mammalian cells and is suitable for creation of
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 2x1,000 to 2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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