M-1

Kidney cell studies, differentiation studies.

Derived from the cortical collecting duct (CCD) of a mouse, transgenic for the early region of SV40 (Tg(SV40E)Bri/7). They express many characteristics of the CCD like epithelial morphology and CCD-specific antigens. M-1 cells grown on permeable supports exhibited a high transepithelial resistance concomitant with the development of a lumen-negative transepithelial potential difference. The M-1 cell line and sub-clones exhibit principal cell (PC) functions and β-intercalated cell (β-ICC) functions, both typical for the heterogeneous epithelium found in the renal collecting duct. M-1 cells and sub-clones show a heterogeneous expression of PC and β-ICC antigens with 5-10% exhibiting a dual PC/β-ICC phenotype.

Mouse kidney cortical collecting duct, SV40 transformed

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 5μm Dexamethasone (DXMT) + 5% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Prolonged incubation with trypsin may be necessary for subculture.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.