产品名称
P-tyr-1, no DNA, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
Mouse x Mouse Hybridoma
manufacturer/tradename
ECACC
packaging
tube of 5 μg 95092903-DNA-5UG, pkg of vial of cells 95092903-1VL
ImmunoDon
BALB/c mouse spleen
Myeloma
P3X63Ag8.653
Reactivity
Anti-Phosphotyrosine
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−196°C
Immunogen
Aminobenzyl phosphonate
Application
Detection of phosphotyrosine-containing proteins
Biochem/physiol Actions
Mouse P3X63Ag8.653 x Mouse BALB/c spleen
The hybridoma cell line P-tyr-1 was produced by fusing P3X63Ag8.653 mouse myeloma cells with spleen cells from a female BALB/c mouse immunised with aminobenzyl phosphonate. Aminobenzyl phosphonate is a structural analogue of the side-chain in phosphotyrosine, and was coupled to hemocyanin (antibody P-HC) for immunisation. The secreted IgG1 antibodies react with phosphotyrosine and will detect phosphotyrosine-containing proteins by immunoprecipitation and Western blot analysis. The antibody does not react with phosphoserine or phosphothreonine. P-tyr-1 was tested for Bovine Viral Diarrhoea Virus (BVDV) at ECACC and found to be negative.
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.