biological source
mouse kidney
packaging
tube of 5 μg 95102517-DNA-5UG, pkg of vial of cells 95102517-1VL
growth mode
Adherent
karyotype
Hyperdiploid
morphology
Epithelial
products
WT1 (expressed gene)
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Gene expression and protein studies connected to kidney development and Wilms′ tumourigenesis.
Biochem/physiol Actions
Mouse mesonephric epithelium, polyoma virus large T transformed
The WT1-expressing mesonephric cell line M15 (alias Meso15) was established from mouse mesonephros transgenically expressing the large T protein of polyoma virus under the control of the early viral enhancer. As a tumour suppresser gene with a key role in urogenital development, WT1 is implicated as predisposition gene in the pathogenesis of Wilms′ tumour (WT).
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 1x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. NB: Cell growth is impaired at lower densities.
Other Notes
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法规信息
低风险生物材料
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