biological source
human lymph
packaging
tube of 5 μg 95111735-DNA-5UG, pkg of vial of cells 95111735-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Bromodeoxyuridine resistant assays
TK-6 cell line has been used to study the in vitro genotoxic effects of trans- and cis-2-ethylhexyl 4-methoxycinnamate (EHMC). It has also been used to study the interaction of silver nanomaterials with the cells.
Biochem/physiol Actions
Human lymphoblast, thymidine kinase heterozygote
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 12,13
D7S820: 9,11
THO1: 8,9.3
TPOX: 8,11
vWA: 17,20
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 12,13
D7S820: 9,11
THO1: 8,9.3
TPOX: 8,11
vWA: 17,20
The thymidine kinase heterozygote cell line TK6 has been isolated from the lymphoblastoid line HH4. HH4 was derived from the WIL-2 cell line. TK6 cells are CD19 positive, 50% of the cell population express CD20 and a small sub-population has been tested positive for CD22. We cannot confirm the BVDV status of this cell line.
Preparation Note
Maintain cultures between 1-9 x 100,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 2mM Glutamine + 10% Horse Serum (HS) or FBS.
Other Notes
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法规信息
常规特殊物品
低风险生物材料
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