biological source
human ovary
packaging
tube of 5 μg 96020759-DNA-5UG, pkg of vial of cells 96020759-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
VEGF
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Human ovarian serous carcinoma (ascites) stage IV, poorly differentiated
STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 8,9
D16S539: 10,13
D5S818: 11,12
D7S820: 10
THO1: 6,9
TPOX: 9,11
vWA: 15,19
CSF1PO: 11
D13S317: 8,9
D16S539: 10,13
D5S818: 11,12
D7S820: 10
THO1: 6,9
TPOX: 9,11
vWA: 15,19
The OV56 cell line was established from ascitic fluid derived from a 46-year-old female patient diagnosed with ovarian cancer. The tumour was characterised as stage IV, poorly differentiated. Immunocytochemical staining of the cells was reported to show strong staining for cytokeratin (CK), weak staining for carcinoembryonic antigen (CEA) and strong staining for human milk fat globulin (HMFG-2). Production of vascular endothelial growth factor (VEGF) has been detected by ELISA. The patient had received sequential treatment with cisplatin and cyclophosphamide, cisplatin and etoposide and subsequently tamoxifen.
Preparation Note
DMEM:HAMS F12 (1:1) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS) + 0.5 ug/ml hydrocortisone + 10ug/ml insulin
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.