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UNSPSC Code:
41106514
Biological source:
Mouse x Mouse Hybridoma
Isotype:
IgG1
Technique(s):
cell culture | mammalian: suitable
Myeloma:
Sp2/0
Reactivity:
Anti-Proteasomes, human
biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 96030418-DNA-5UG, pkg of vial of cells 96030418-1VL
ImmunoDon
BALB/c mouse spleen
Myeloma
Sp2/0
Reactivity
Anti-Proteasomes, human
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−196°C
Immunogen
Human placenta proteasomes
Application
Detection and purification of proteasomes and analysis of subunits
Biochem/physiol Actions
Antibodies secreted by the hybridoma line MCP21 react with subunit C3 in human and rabbit proteasomes, both with intact proteasomes and on blots. The hybridoma has been produced by repeated intraperitoneal immunisation of BALB/c mice with 20-50 μg of human placenta proteasomes and subsequent fusion of spleen cells with myeloma Sp2/0.
Mouse Sp2/0 x Mouse BALB/c spleen
Preparation Note
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Maintain cultures between 5x10,000 and 1x1,000,000 cells/ml; 5% CO2; 37°C
Other Notes
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法规信息
低风险生物材料
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