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Merck
CN

EMT6/VRP/R

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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关于此项目

UNSPSC Code:
41106514
Biological source:
mouse unknown/unspecified
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Epithelial-like

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biological source

mouse unknown/unspecified

packaging

tube of 5 μg 96042319-DNA-5UG, pkg of vial of cells 96042319-1VL

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial-like

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

相关类别

Application

Drug-resistance studies

Biochem/physiol Actions

Mouse mammary, drug-resistant
The drug-resistant line EMT6/VRP/R was established from the parent cell line EMT6/P (Sigma Catalogue number. 96042344) by growth in verapamil (see reference).

Preparation Note

MEM (EBSS) +2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 75 μg/ml verpamil + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO2;37ºC. It is recommended to resuscitate cells in drug-free medium. Add drug after growth is established.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

法规信息

低风险生物材料
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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