biological source
human lung
packaging
tube of 5 μg 96042329-DNA-5UG, pkg of vial of cells 96042329-1VL
growth mode
Aggregates in suspension
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Analysis of acquired drug-resistance, study of small cell lung cancer
Biochem/physiol Actions
Human Caucasian small cell lung carcinoma, drug-resistant
NCI-H69/LX4 has been established by exposure of the parent line, H69 (Sigma Catalogue number. 91091802) to doxorubicin, also known as adriamycin. The line hyperexpresses P-glycoprotein and demonstrates a multidrug resistant drug accumulation deficit. It is recommended to culture the cells without drug after resuscitation until the first passage.
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 12
D16S539: 11
D5S818: 11,13
D7S820: 9
THO1: 8,9
TPOX: 10
vWA: 16,17
CSF1PO: 10,12
D13S317: 12
D16S539: 11
D5S818: 11,13
D7S820: 9
THO1: 8,9
TPOX: 10
vWA: 16,17
Preparation Note
RPMI + 2mM Glutamine + 0.4μg/ml doxorubicine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) by repeated pipetting of aggregates or scraping; 5% CO2; 37°C
Other Notes
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法规信息
常规特殊物品
低风险生物材料
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