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eCl@ss:
32190102
UNSPSC Code:
41106514
Biological source:
human lung
Relevant disease(s):
cancer
Growth mode:
Aggregates in suspension
Karyotype:
Not specified
Morphology:
Not specified
biological source
human lung
packaging
tube of 5 μg 96042330-DNA-5UG, pkg of vial of cells 96042330-1VL
growth mode
Aggregates in suspension
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Drug-resistance studies
Biochem/physiol Actions
Human small cell lung cancer, drug-resistant
NCI-H69 VCR/R has been derived from the parent small cell lung cancer line NCI-H69 (Sigma Catalogue number. 91091802) It is recommended to culture cells without drug upon resuscitation; add vincristine once growth is fully established. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 11
D5S818: 11
D7S820: 9
THO1: 8,9
TPOX: 10
vWA: 16,17
CSF1PO: 12
D13S317: 12
D16S539: 11
D5S818: 11
D7S820: 9
THO1: 8,9
TPOX: 10
vWA: 16,17
Preparation Note
Maintain cultures between 3-9x 100,000 cells/ml; 5% CO2; 37ºC. Resuscitate in drug-free medium, add after growth has been established.
RPMI 1640 + 2mM Glutamine + 2.0 μg/ml vincristine + 10% Foetal Bovine Serum (FBS).
Other Notes
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法规信息
常规特殊物品
低风险生物材料
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