MOR/CPR
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 96042333, human lung, Not specified
生物来源
human lung
包装
tube of 5 μg 96042333-DNA-5UG
pkg of vial of cells 96042333-1VL
生长模式
Aggregates in suspension
核型
Not specified
形态学
Not specified
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
应用
Analysis of acquired drug resistance
生化/生理作用
Human lung adenocarcinoma, drug-resistant
MOR/CPR has been developed by growing the parent line, MOR, in increasing concentrations of cisplatin. The cells are cross-resistant to melphalan but show little or no cross-resistance to other platinum compounds. Cisplatin accumulation has been found to be reduced compared to the parent line.
制备说明
RPMI 1640 + 2mM Glutamine + 1μg/ml cisplatin + 10% Foetal Bovine Serum (FBS).
Split subconfluent cultures (70-80%) 1:2 to 1:4 seeding at 2-4 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. It is recommended to culture the cells without drug after resuscitation until the first passage. These cells produce a
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
法规信息
高风险级别生物产品--人源产品
动植物来源培养基
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