biological source
human lung
packaging
tube of 5 μg 96042336-DNA-5UG, pkg of vial of cells 96042336-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Analysis of acquired drug resistance, study of large cell carcinoma of the lung
Biochem/physiol Actions
Human Caucasian lung, large cell carcinoma
STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 10,11
D16S539: 11,13
D5S818: 10,12
D7S820: 9
THO1: 9
TPOX: 8
vWA: 19
CSF1PO: 11
D13S317: 10,11
D16S539: 11,13
D5S818: 10,12
D7S820: 9
THO1: 9
TPOX: 8
vWA: 19
The cell line COR-L23/CPR is a drug-resistant variant of COR-L23 (Sigma Catalogue number. 92031919). The line was developed by growing the parent line in increasing concentrations of cisplatin. Cisplatin accumulation is reduced in COR-L23/CPR and cross resistance to melphalan and other platinum compounds has been found. Cells tend to grow in clumps.
Preparation Note
RPMI 1640 + 2mM Glutamine + 0.05μg / ml Cisplatin + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 2-5 x 10,000 cells / cm2 using 0.25 % trypsin or trypsin/EDTA; 5% CO2; 37°C. It is recommended to culture the cells without drug upon resuscitation until the first passage.
Other Notes
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法规信息
低风险生物材料
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