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OE33 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 96070808, human esophagus, Epithelial

别名:

JROECL33, JROECL33 Cells, OE-33 Cells

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UNSPSC代码:
41106514

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生物来源

human esophagus

包装

tube of 5 μg 96070808-DNA-5UG
pkg of vial of cells 96070808-1VL

生长模式

Adherent

核型

Aneuploid

形态学

Epithelial

产品

Not specified

受体

MHC class I, ICAM-1

技术

cell culture | mammalian: suitable

相关疾病

cancer

运输

dry ice

储存温度

−196°C

相关类别

应用

OE33 cell line has been used to study the effect of microRNA-MiR-193b on autophagy and cell death on these cells. It has also been used to study the sensitivity of the cells to anti-c-Met monoclonal antibody ABT-700.
Study of oesophageal cancer

生化/生理作用

Human Caucasian oesophageal carcinoma
The cell line OE33, also known as JROECL33, was established from the adenocarcinoma of the lower oesophagus (Barrett′s metaplasia) of a 73 year old female patient. The tumour was identified as pathological stage IIA (UICC) and showed poor differentiation. OE33 express HLA-A, -B and -C antigens (MHC class I) and ICAM-1 constitutively. Expression of HLA-DR (MHC class II) can be induced by treatment with interferon-gamma. The cells express epithelial cytokeratins and are tumourigenic in nude mice.

制备说明

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.
Split sub-confluent cultures (70-80%) 1:8 i.e. seeding at 1x10,000 cells/cm2 using 0.25% Trypsin or Trypsin/EDTA; 5% CO2; 37°C. Initially these cells grow slowly and can take up to 7 days until ready for the next split, 50% media changes will be necessary every 2-3 days (i.e. replacing half the old medium with fresh).

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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  1. How do I handle Cell Line frozen cells upon arrival?

    Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.

  2. Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?

    If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.

  3. How do I resuscitate/thaw frozen Cell Line cells?

    A protocol for thawing cells can be found in the ECACC handbook.

  4. What medium should I use when I thaw the Cell Line cells?

    The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.

  5. What passage are my Cell Line cells?

    If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).

  6. How many Cell Line cells are in the vial?

    Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule.  For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.

  7. Are my Cell Line cells mycoplasma tested?

    ECACC routinely tests all manufactured cell banks for mycoplasma.

  8. How to freeze down Cell Line cells to make a cell stock?

    A protocol for freezing down cells for cell stocks can be found in the ECACC manual.

  9. Are the Cell Line cells tested for viral pathogens?

    HPA Cultures does not perform any viral testing on the cell lines.

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