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关于此项目
UNSPSC Code:
41106514
Biological source:
human blood
Relevant disease(s):
cancer
Growth mode:
Suspension
Karyotype:
92XXXX/69XXX,t(1,2)(p32,q34)t(2,8)(p11,q24)t(14,18
Morphology:
Lymphoblastoid
biological source
human blood
packaging
tube of 5 μg 96090512-DNA-5UG, pkg of vial of cells 96090512-1VL
growth mode
Suspension
karyotype
92XXXX/69XXX,t(1,2)(p32,q34)t(2,8)(p11,q24)t(14,18
morphology
Lymphoblastoid
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Study of B-cell lymphoma-leukaemia
Biochem/physiol Actions
Human Caucasian B-cell lymphoma
The cell line Ri-1 has been established from the peripheral blood of a 48-year-old female patient with B-cell lymphoma. This EBV-negative cell line with 14q+ abnormality was shown to be at an intermediate stage of B-cell differentiation. Ri-1 expresses surface and cytoplasmic immunoglobulin (IgM kappa), HLA-DR, CD20, CD38 and FMC7. Rearranged heavy-light chain genes and one rearranged kappa light-chain gene was detected as well as a kappa and lambda light-chain gene in germ-line configuration.
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cell growth is very slow after resuscitation; large amounts of debris present.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
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法规信息
低风险生物材料
常规特殊物品
此项目有
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