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UNSPSC Code:
41106514
Biological source:
Mouse x Mouse Hybridoma
Isotype:
IgG1
Technique(s):
cell culture | mammalian: suitable
Myeloma:
P3X63Ag8.653
Reactivity:
Anti-Thioredoxin, human
biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 97011602-DNA-5UG, pkg of vial of cells 97011602-1VL
ImmunoDon
BALB/c mouse spleen
Myeloma
P3X63Ag8.653
Reactivity
Anti-Thioredoxin, human
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−196°C
Immunogen
Human Thioredoxin cross linked to Keyhole Limpet Haemocyanin
Application
Immunohistochemistry, Western blots
Biochem/physiol Actions
BALB/c mouse spleen x Mouse P3X63Ag8.653
CAR-2/3F4, also known as 2/3 F4, was formed by fusing P3X63Ag8-653 mouse myeloma cells with spleen cells from a BALB/c mouse immunised six times with 20μg human thioredoxin (TRX) at monthly intervals. Human TRX was cross-linked to Keyhole Limpet Haemocyanin for this purpose. The antibody is directed against monomeric and polymeric forms of human TRX and shows no cross-reactivity to bacterial TRX or human glutaredoxin. CAR-2/3F4 has been applied as capture antibody in a chemiluminescent detection assay for free human TRX. CAR-XP10 (Sigma Catalogue number. 97011601) can be used as second antibody in this assay.
Preparation Note
Maintain cultures between 2-9 x 100,000 cells / ml; 5% CO2; 37°C; start culture on mouse macrophage feeder layer
RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 15% Foetal Bovine Serum (FBS)
Other Notes
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法规信息
低风险生物材料
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