IgG-6A6

VLDL peptide coupled to keyhole limpet hemocymin

Immunoblot analysis of distribution and regulation of the VLDL receptor in animal tissues and cultured cells.

BALB/c mouse spleen x Sp2/0

IgG-6A6 secretes a mouse monoclonal antibody that binds to the cytoplasmic domain of the very low density lipoprotein (VLDL) receptor. The hybridoma was produced by intraperitoneal injection of a peptide corresponding to the 10 carboxy-terminal amino acids of the VLDL receptor (SVVSTDDDLA) being coupled to keyhole limpet hemocyanin. BALB/c mice spleen cells were then fused with Sp2/0 mouse myeloma for the detection of VLDL receptor in various tissues.

DMEM + 2mM glutamine + 10% FBS

Maintain cultures between 1-9 x 100,000 cells/ml; 5% CO₂; 37°C; media renewal every 2-3 days

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.