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关于此项目
eCl@ss:
32190102
UNSPSC Code:
41106514
Biological source:
rat lymph
Relevant disease(s):
cancer
Growth mode:
Suspension
Karyotype:
42XY,-17,t(2,4)(q22,q24),t(10,11)(q12,q12),+der(15)t(15,?)(p12,?),-18,+der(18)t(18,?)(p12,?)
Morphology:
Lymphoblast
biological source
rat lymph
packaging
tube of 5 μg 97041101-DNA-5UG, pkg of vial of cells 97041101-1VL
growth mode
Suspension
karyotype
42XY,-17,t(2,4)(q22,q24),t(10,11)(q12,q12),+der(15)t(15,?)(p12,?),-18,+der(18)t(18,?)(p12,?)
morphology
Lymphoblast
products
Not specified
receptors
Modified prolactin receptor (the Nb2 form)
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
1) in vitro bioassay of lactogenic hormones.
2) Studies of the mechanisms of lactogen induced mitogenesis.
3) Studies into the action of vinca alkaloids.
4) Studies of tumour progression.
2) Studies of the mechanisms of lactogen induced mitogenesis.
3) Studies into the action of vinca alkaloids.
4) Studies of tumour progression.
Biochem/physiol Actions
Nb2-11 is a clone of the Nb-2 rat lymphoma line which was derived from a transplant of a lymphoma that developed in the thymus/lymph node of a male noble (Nb) strain rat following prolonged oestrogen treatment. The cells are of the pre-T cell origin and their proliferation is dependent on mammalian lactogens, such as prolactin. Nb2-11 can also be mitogenically stimulated by IL-2. Injection of Nb2 cells into Nb rats gives rise to malignant tumours that are highly sensitive to treatment with vinca alkaloids. Karyotypic analysis has shown that the cell line has only five well developed chromosome abnormalities. The cells do not express surface immunoglobulin, and their lactogen dependency was confirmed before deposit. Protocols for the use of Nb2-11 cells in bioassays are available from ECACC on request.
Rat lymphoma
Preparation Note
At resuscitation seed cultures at 3-5 x 100,000 cells/ml. Once established cultures should be seeded at 4 x 1,000 and 12 x 1,000 cells/ml for culture periods of 96 and 72 hours respectively. Do not exceed 9 x 100,000 cells/ml. Avoid using dilutions of cells with fresh medium at greater than 1:10 ratios; culture doubling time 12 hours.
Fischer′s medium + 10% Fotal Bovine Serum (FBS) + 10% Horse Serum (HS) (gelding) + 0.075% Sodium Bicarbonate + 0.05 mM 2-Mercaptoethanol (2ME) + 2 mM Glutamine.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.