MFE-296
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 98031101, human endometrium, Epithelial
别名:
MFE296 Cells
生物来源
human endometrium
包装
tube of 5 μg 98031101-DNA-5UG
pkg of vial of cells 98031101-1VL
生长模式
Adherent
核型
Near Tetraploid
形态学
Epithelial
产品
Not specified
受体
Androgen receptor
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
应用
Tumour biology studies, receptor analysis
生化/生理作用
Human caucasian endometrial adenocarcinoma
MFE-296 was obtained from a moderately differentiated human endometrial adenocarcinoma. The cells were shown to express functionally intact androgen receptors. Proliferation was inhibted by progestin R5020 and dihydrotestosterone (DHT), the latter one being antagonised by the antiandrogen Casodex. MFE-296 express vimentin and the cytokeratins 7, 8, 18 and 19. Karyotype analysis revealed near tetraploidy for most cells. Further more, the cells have been shown to be tumorigenic in nude mice.
STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 8,13
D16S539: 11,14
D5S818: 11,13
D7S820: 8,10
THO1: 6,9.3
TPOX: 8
vWA: 14,18
CSF1PO: 10
D13S317: 8,13
D16S539: 11,14
D5S818: 11,13
D7S820: 8,10
THO1: 6,9.3
TPOX: 8
vWA: 14,18
制备说明
MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:4 i.e. seeding 1-2 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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