Androgen receptor studies, study of mammary carcinoma

Human caucasian mammary carcinoma

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 8,12
D16S539: 11
D5S818: 11,12
D7S820: 8,10
THO1: 6,9.3
TPOX: 8
vWA: 17

The mammary carcinoma cell line MFM-223 was obtained from the pleural effusion of a postmenopausal breast cancer patient. The patient had not received any prior treatment and the tumour was classified as a widespread ductal mammary carcinoma with the histological grade of III. The cell line is characterised by high androgen, (approximately 100,000 binding sites per cell) and low oestrogen and progesterone receptor levels. Proliferation of cells was inhibited by addition of the androgen dihydrotestosterone at concentrations above 0.01 nM. MFM-223 have a characteristic pattern of isoenzyme polymorphism and of karyotype alterations revealing marker chromosomes and homogenously staining regions. Most cells have been near diploid with 10% displaying near tetraploidy. The cells have been tested to be free of Hepatitis B and C viruses and HIV. MFM-223 is tumourigenic in nude mice.

MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.