MFE-280

Tumour biology studies

Human caucasian endometrial adenocarcinoma

MFE-280 was derived from a recurrent, poorly differentiated endometrial carcinoma from a 78 year old patient. Initially, cells were grown as aggregates in suspension for one year, after which they were transferred to monolayer culture. The karyotype analysis revealed near-diploidy with a complex heterogeneous aberration pattern. MFE-280 were found to be positive for cytokines 7, 8, 18 and 19 as well as for vimentin. In addition, the cells express the placental protein 14, PP14. They have been shown to be tumorigenic in nude mice.

STR-PCR Data: Amelogenin: X
CSF1PO: 9,12
D13S317: 10,12
D16S539: 12
D5S818: 11,12
D7S820: 10
THO1: 7
TPOX: 9,10
vWA: 17

MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding 2-4 x 10,000 cells / cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.