biological source
human endometrium
packaging
tube of 5 μg 98050131-DNA-5UG, pkg of vial of cells 98050131-1VL
growth mode
Adherent
karyotype
Near diploid
morphology
Epithelial
products
Placental protein 14
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumour biology studies
Biochem/physiol Actions
Human caucasian endometrial adenocarcinoma
MFE-280 was derived from a recurrent, poorly differentiated endometrial carcinoma from a 78 year old patient. Initially, cells were grown as aggregates in suspension for one year, after which they were transferred to monolayer culture. The karyotype analysis revealed near-diploidy with a complex heterogeneous aberration pattern. MFE-280 were found to be positive for cytokines 7, 8, 18 and 19 as well as for vimentin. In addition, the cells express the placental protein 14, PP14. They have been shown to be tumorigenic in nude mice.
STR-PCR Data: Amelogenin: X
CSF1PO: 9,12
D13S317: 10,12
D16S539: 12
D5S818: 11,12
D7S820: 10
THO1: 7
TPOX: 9,10
vWA: 17
CSF1PO: 9,12
D13S317: 10,12
D16S539: 12
D5S818: 11,12
D7S820: 10
THO1: 7
TPOX: 9,10
vWA: 17
Preparation Note
MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding 2-4 x 10,000 cells / cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C
Other Notes
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法规信息
低风险生物材料
常规特殊物品
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