biological source
human blood
packaging
tube of 5 μg 98062313-DNA-5UG, pkg of vial of cells 98062313-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
DNA repair studies
Biochem/physiol Actions
Derived from a 58 year old normal male. The mononuclear cells have been used as a normal control for DNA repair deficiency studies, effect of UV irradiation and hprt mutation analysis. They have subsequently been transformed with EBV.
Human lymphocytes, normal, EBV-transformed
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11
D13S317: 8
D16S539: 11,12
D5S818: 11
D7S820: 10,12
THO1: 7,9
TPOX: 8,11
vWA: 14,16
CSF1PO: 11
D13S317: 8
D16S539: 11,12
D5S818: 11
D7S820: 10,12
THO1: 7,9
TPOX: 8,11
vWA: 14,16
Preparation Note
Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO2, 37°C. Cells form floating aggregates.
RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.