biological source
human blood
packaging
tube of 5 μg 98062318-DNA-5UG, pkg of vial of cells 98062318-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Mutation studies
Biochem/physiol Actions
Human lymphoblastoid, normal,EBV-transformed
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11
D16S539: 9
D5S818: 10,13
D7S820: 11
THO1: 8,9
TPOX: 8,10
vWA: 17,18
CSF1PO: 11,12
D13S317: 11
D16S539: 9
D5S818: 10,13
D7S820: 11
THO1: 8,9
TPOX: 8,10
vWA: 17,18
The lymphocyte cell line LB185 has been established using mononuclear cells from peripheral blood of a 56 year old normal male. T cells from the same sample have been used as controls for cellular radiosensitivity studies. Cells have been subsequently transformed with EBV. Fibroblasts have been derived from the same individual and were designated 153BR (Sigma Catalogue number. 90011808). This has been confirmed by STR profiling at ECACC. Cells are lymphhoblastoid with floating aggregates present.
Preparation Note
Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO2, 37°C. Cells form floating aggregates.
RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).
Other Notes
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法规信息
常规特殊物品
低风险生物材料
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