biological source
human blood
packaging
tube of 5 μg 98062320-DNA-5UG, pkg of vial of cells 98062320-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
DNA repair deficiency studies
Biochem/physiol Actions
Human lymphocytes, Trichothiodystrophy heterozygote, EBV-transformed
LB198 has been derived from mononuclear cells from peripheral blood of a 45 year old female. The cells have been transformed with EBV. The patient has been shown to be heterozygote for trichothiodystrophy (TTD) and is the parent of LB199 (Sigma Catalogue number.98062321) TTD is a rare genetic disorder with deficiency in excision repair of UV damage. Fibroblasts from the same individual have been designated TTD HM 1BR.
Preparation Note
Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO2, 37°C. Cells form floating aggregates.
RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).
Other Notes
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法规信息
常规特殊物品
低风险生物材料
此项目有
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