登录 查看组织和合同定价。
选择尺寸
关于此项目
UNSPSC Code:
41106514
Biological source:
human lymph (axillary)
Relevant disease(s):
cancer
Growth mode:
Suspension
Karyotype:
Hyperdiploid, modal no. 47, chromosome 1 trisomy, 15 monosomy, +14q+
Morphology:
Lymphoblastoid
biological source
human lymph (axillary)
packaging
tube of 5 μg 98070109-DNA-5UG, pkg of vial of cells 98070109-1VL
growth mode
Suspension
karyotype
Hyperdiploid, modal no. 47, chromosome 1 trisomy, 15 monosomy, +14q+
morphology
Lymphoblastoid
products
Not specified
receptors
sIgM,CD22,BB1,HLA-ABC,GnRH
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
B lymphoma studies, cancer treatments
Biochem/physiol Actions
Human B-type EBNA negative lymphoma
The cell line BHL-89 was established from a 56 year old female patient with chronic lymphoid leukaemia (CLL). The B-cell type cells were shown to express CD9, CD22, CD71,HLA-ABC, BB1, GnRH and surface IgM. Cloning efficiency in soft agar was found to be between 20-60% and population doubling time was 48 hours. BHL-89 cells are Epstein-Barr nuclear antigen (EBNA) negative. They have been shown to be tumourigenic in immunosuppressed mice, sensitive to dibromodulcitol and resistant to the phorbol ester TPA. The cell line was found to be positive for BCL-2 protein and produces small amounts of interferon. Lymphoblastoid cells and floating aggregates. The cell line has been eradicated from mycoplasma at ECACC.
Preparation Note
Maintain cultures between 1-5 x 100,000 cells/ml;5% CO2; 37°C
RPMI1640 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
法规信息
常规特殊物品
低风险生物材料
此项目有
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持