生物来源
human lymph (axillary)
包装
tube of 5 μg 98070109-DNA-5UG
pkg of vial of cells 98070109-1VL
生长模式
Suspension
核型
Hyperdiploid, modal no. 47, chromosome 1 trisomy, 15 monosomy, +14q+
形态学
Lymphoblastoid
产品
Not specified
受体
sIgM,CD22,BB1,HLA-ABC,GnRH
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
应用
B lymphoma studies, cancer treatments
生化/生理作用
Human B-type EBNA negative lymphoma
The cell line BHL-89 was established from a 56 year old female patient with chronic lymphoid leukaemia (CLL). The B-cell type cells were shown to express CD9, CD22, CD71,HLA-ABC, BB1, GnRH and surface IgM. Cloning efficiency in soft agar was found to be between 20-60% and population doubling time was 48 hours. BHL-89 cells are Epstein-Barr nuclear antigen (EBNA) negative. They have been shown to be tumourigenic in immunosuppressed mice, sensitive to dibromodulcitol and resistant to the phorbol ester TPA. The cell line was found to be positive for BCL-2 protein and produces small amounts of interferon. Lymphoblastoid cells and floating aggregates. The cell line has been eradicated from mycoplasma at ECACC.
制备说明
Maintain cultures between 1-5 x 100,000 cells/ml;5% CO2; 37°C
RPMI1640 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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