biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 99043002-DNA-5UG, pkg of vial of cells 99043002-1VL
ImmunoDon
BALB/c X C57bL/6)F1,spleen
Myeloma
P3x63 Ag6.653
Reactivity
Anti-HLA DR13
technique(s)
cell culture | mammalian: suitable
isotype
IgM
shipped in
dry ice
storage temp.
−196°C
Immunogen
B cell line Sch HLA A2, B44, C5 DR13
Application
Serological and biochemical analysis of class II products encoded by HLA-DRw6 haplotype
Biochem/physiol Actions
P3 x 63 Ag8.653
The hybridoma line S2 has been obtained by fusion of the myeloma P3x63 Ag6.653 and spleen cells from (C57 BL/6 x BALB/c) F1 female mice after a single i.p. injection of lymphoblastoid cells. These lymphoblastoid cells were DRw6 homozygous and expressed HLA A2, Bw44, CW5, DRw6 and 16.23+. The monoclonal antibody secreted by S2 defines an epitope apparently restricted to DRQ13. Together with antibodies expressed by 16/23 (Sigma Catalogue number. 99043001) and S5 (Sigma Catalogue number. 99061827) they are useful tools for the biochemical dissection of DRw6 related epitopes. S2 has been analysed in International Histocompatibility Workshop (IOWS 3021, II WS 0826). The cells have been eradicated from mycoplasma before depositing at ECACC. The cells have a doubling time of about 36 hours.
Preparation Note
Maintain cultures between 3-9 x 100,000 cells/ml, 5% CO2; 37°C.
RPMI 1640 + 2mM glutamine + 10% FBS
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.