biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 99043003-DNA-5UG, pkg of vial of cells 99043003-1VL
ImmunoDon
BALB/c x C57 Bl/6
Myeloma
P3x63Ag8.653
Reactivity
Anti-DR11
technique(s)
cell culture | mammalian: suitable
isotype
IgM
shipped in
dry ice
storage temp.
−196°C
Immunogen
B cell line LuMar
Application
Immunofluorescence, cytotoxicity, immunoprecipitation
Biochem/physiol Actions
Mouse x Mouse hybridoma
The cell line Lu2 has been established by fusion of the myeloma P3x63Ag8.653 and spleen cells from BALB/c x C57Bl/6 female mice. Mice received a single i.p. injection with the B cell line LuMar expressing HLA A2.3/B18.5/C5/DR3,11.and B. pertussis three days before fusion. The cell line has been eradicated from mycoplasma using Cibrobay.
Preparation Note
Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37°C
RPMI 1640 + 2mM glutamine + 10% FBS
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.
法规信息
动物来源培养基
此项目有