C1

B cell line Co, HLA A1,B8 C7, DR3,DQ2

Immunofluorescence, cytotoxicity

Mouse x Mouse hybridoma

The hybridoma C1 was produced from a (C57Bl/6xBALB/c)F1 mouse immunised with a single i.p. injection of the lymphoblastoid cell line Co and Bordetella pertussis organisms. The B cell line Co was expressing HLA A1, B8, C7, DR3 and DQ2. The mouse spleen was removed 3 days after immunisation and fused with the myeloma P3x63Ag8.653. C1 monoclonal antibodies were shown to react with cells expressing the class II antigen DQw2. The hybridoma is also known as COX IA2. C1 has been analysed in the International Histocompatibility Workshop 10 (IOW3105) and 12 (12W593).

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37°C

RPMI 1640 + 2mM glutamine + 1mM NaP + 10% FBS

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