biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 99061825-DNA-5UG, pkg of vial of cells 99061825-1VL
ImmunoDon
(C57BL/6xBALB/c)F1
Myeloma
P3x63Ag8.653
Reactivity
Anti-HLA DQ2
technique(s)
cell culture | mammalian: suitable
isotype
IgM
shipped in
dry ice
storage temp.
−196°C
Immunogen
B cell line Co, HLA A1,B8 C7, DR3,DQ2
Application
Immunofluorescence, cytotoxicity
Biochem/physiol Actions
Mouse x Mouse hybridoma
The hybridoma C1 was produced from a (C57Bl/6xBALB/c)F1 mouse immunised with a single i.p. injection of the lymphoblastoid cell line Co and Bordetella pertussis organisms. The B cell line Co was expressing HLA A1, B8, C7, DR3 and DQ2. The mouse spleen was removed 3 days after immunisation and fused with the myeloma P3x63Ag8.653. C1 monoclonal antibodies were shown to react with cells expressing the class II antigen DQw2. The hybridoma is also known as COX IA2. C1 has been analysed in the International Histocompatibility Workshop 10 (IOW3105) and 12 (12W593).
Preparation Note
Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37°C
RPMI 1640 + 2mM glutamine + 1mM NaP + 10% FBS
Other Notes
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法规信息
低风险生物材料
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