F-36P

Study of differentiation and effect of growth signals

Human leukemia, myelodysplastic syndrome

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 9
D13S317: 10
D16S539: 10,12
D5S818: 13
D7S820: 9,11
THO1: 8
TPOX: 9,11
vWA: 14,16

The cell line F-36P has been established from a 65 year old male patient, who was diagnosed with refractory anaemia with excess blasts (RAEB), a subtype of myelodysplastic syndrome (MDS). This non-lymphoid leukemia line was obtained from the patient′s pleural fluid at time of progressed to overt leukemia. F-36P is cytokine-dependent requiring granulocyte-macrophage colony-stimulating factor (GM-CSF) or Interleukin 3 (IL-3). Cells have been shown to synthesize haemoglobin when GM-CSF or IL-3 is substituted by erythropoietin (Epo). They have been reported to be positive for leukocyte common antigen (CD45) and some multilineage markers such as CD13, CD33 and CD34, but are negative for T- and B-cell antigens and mature myelomonocytic antigens. Due to their reaction with some monoclonal antibodies recognising erythroid and platelet glycoproteins it has been suggested that F-36P has a multilineage phenotype.

Maintain cultures between 3-9 x 100,000 cells/ml; 5%CO₂; 37ºC.

RPMI 1640 + 2mM Glutamine + 5ng/ml rh IL-3 + 5% Foetal Bovine Serum (FBS).

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