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MC3T3-E1 Cell Line from mouse

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 99072810, mouse bone, Fibroblast-like

别名:

MC3T3E1 Cells

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关于此项目

eCl@ss:
32190102
UNSPSC Code:
41106514
Biological source:
mouse bone
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Fibroblast-like

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biological source

mouse bone

description

Mouse C57BL/6 calvaria

packaging

tube of 5 μg 99072810-DNA-5UG, pkg of vial of cells 99072810-1VL

growth mode

Adherent

karyotype

Not specified

morphology

Fibroblast-like

products

Collagen

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

相关类别

Application

MC3T3-E1 Cell Line has been used to study and propose an actin filament cytoskeleton analysis framework. It has also been used to study the effect of biodegradable magnesium and magnesium alloys on selected properties of these cells stimulated by direct cell/material interaction.

Biochem/physiol Actions

Mouse C57BL/6 calvaria
The osteoblastic cell line MC3T3-E1 has been established from a C57BL/6 mouse calvaria and selected on the basis of high alkaline phosphatase (ALP) activity in the resting state. Cells have the capacity to differentiate into osteoblasts and osteocytes and have been demonstrated to form calcified bone tissue in vitro. Mineral deposits have been identified as hydroxyapatite. Expression of basic fibroblast growth factor (bFGF) mRNA and protein has been shown to be regulated by treatment with TGF β and bFGF. Prostaglandin F2a has been reported to stimulate DNA synthesis and proliferation by up-regulation of insulin-like growth factor I receptors. MC3T3-E1 secrete collagen and express murine leukemia inhibitory factor (mLIF) in RNA.

Preparation Note

MEM α + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:4 using 0.25% trypsin or trypsin/EDTA 5% CO2 37ºC.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

法规信息

低风险生物材料
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商品

ECACC Nucleic Acids

DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.

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