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About This Item

UNSPSC代码:
41106514
eCl@ss:
32190102
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生物来源

mouse bone

描述

Mouse C57BL/6 calvaria

包装

tube of 5 μg 99072810-DNA-5UG
pkg of vial of cells 99072810-1VL

生长模式

Adherent

核型

Not specified

形态学

Fibroblast-like

产品

Collagen

技术

cell culture | mammalian: suitable

运输

dry ice

储存温度

−196°C

细胞系来源

Mouse C57BL/6 calvaria

细胞系描述

The osteoblastic cell line MC3T3-E1 has been established from a C57BL/6 mouse calvaria and selected on the basis of high alkaline phosphatase (ALP) activity in the resting state. Cells have the capacity to differentiate into osteoblasts and osteocytes and have been demonstrated to form calcified bone tissue in vitro. Mineral deposits have been identified as hydroxyapatite. Expression of basic fibroblast growth factor (bFGF) mRNA and protein has been shown to be regulated by treatment with TGF β and bFGF. Prostaglandin F2a has been reported to stimulate DNA synthesis and proliferation by up-regulation of insulin-like growth factor I receptors. MC3T3-E1 secrete collagen and express murine leukemia inhibitory factor (mLIF) in RNA.

应用

MC3T3-E1 Cell Line has been used to study and propose an actin filament cytoskeleton analysis framework. It has also been used to study the effect of biodegradable magnesium and magnesium alloys on selected properties of these cells stimulated by direct cell/material interaction.

培养基

MEM α + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:4 using 0.25% trypsin or trypsin/EDTA 5% CO2 37ºC.

其他说明

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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