form
solution (in 10 mM Tris, 1 mM EDTA)
packaging
pkg of 1 vial
pH range
7.5-8.5
shipped in
ambient
storage temp.
−20°C
General description
The US-2019-nCoV N2 Individual Assay is for qPCR (5′ nuclease assay) detection of SARS-CoV-2. The product is primers and probes, the sequences of which were designed and made publicly available by the United States Centers for Disease Control and Prevention (U.S. CDC).
For additional details, please visit SigmaAldrich.com/ncov.
For additional details, please visit SigmaAldrich.com/ncov.
Features and Benefits
The forward and reverse primers in each tube come with the following features:
The Dual-Labeled Probe in each tube comes with the following features:
- DNA bases
- No modifications
- Desalt purified
- 10 nmol each
The Dual-Labeled Probe in each tube comes with the following features:
- DNA bases
- 6-FAM™ reporter
- BXQ (Block All Quencher—SigmaAldrich.com/bxq)
- HPLC purified
- 2.5 nmol
Other Notes
The product includes 1 tube of a 20X primer and probe pool for quantification of a SARS-CoV-2 gene. Assay included is N2.
The forward primer, reverse primer, and probe are at final concentrations of 10, 10, and 2.5 μM, respectively.
N2 Assay:
Forward Primer TTACAAACATTGGCCGCAAA
Reverse Primer GCGCGACATTCCGAAGAA
Dual-Labeled Probe ACAATTTGCCCCCAGCGCTTCAG
The forward primer, reverse primer, and probe are at final concentrations of 10, 10, and 2.5 μM, respectively.
N2 Assay:
Forward Primer TTACAAACATTGGCCGCAAA
Reverse Primer GCGCGACATTCCGAAGAA
Dual-Labeled Probe ACAATTTGCCCCCAGCGCTTCAG
Legal Information
FAM is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable