US-2019-nCoV N2 for detection of SARS-CoV-2 RNA

The US-2019-nCoV N2 Individual Assay is for qPCR (5′ nuclease assay) detection of SARS-CoV-2. The product is primers and probes, the sequences of which were designed and made publicly available by the United States Centers for Disease Control and Prevention (U.S. CDC).

For additional details, please visit SigmaAldrich.com/ncov.

The forward and reverse primers in each tube come with the following features:

• DNA bases
• No modifications
• Desalt purified
• 10 nmol each

The Dual-Labeled Probe in each tube comes with the following features:

• DNA bases
• 6-FAM^™ reporter
• BXQ (Block All Quencher—SigmaAldrich.com/bxq)
• HPLC purified
• 2.5 nmol

The product includes 1 tube of a 20X primer and probe pool for quantification of a SARS-CoV-2 gene. Assay included is N2.

The forward primer, reverse primer, and probe are at final concentrations of 10, 10, and 2.5 μM, respectively.

N2 Assay:

Forward Primer TTACAAACATTGGCCGCAAA
Reverse Primer GCGCGACATTCCGAAGAA
Dual-Labeled Probe ACAATTTGCCCCCAGCGCTTCAG

FAM is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries