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Merck
CN

CDA00011B

US-2019-nCoV N2 for detection of SARS-CoV-2 RNA

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form

solution (in 10 mM Tris, 1 mM EDTA)

packaging

pkg of 1 vial

pH range

7.5-8.5

shipped in

ambient

storage temp.

−20°C

Features and Benefits

The forward and reverse primers in each tube come with the following features:

  • DNA bases
  • No modifications
  • Desalt purified
  • 10 nmol each


The Dual-Labeled Probe in each tube comes with the following features:

  • DNA bases
  • 6-FAM reporter
  • BXQ (Block All Quencher—SigmaAldrich.com/bxq)
  • HPLC purified
  • 2.5 nmol

General description

The US-2019-nCoV N2 Individual Assay is for qPCR (5′ nuclease assay) detection of SARS-CoV-2. The product is primers and probes, the sequences of which were designed and made publicly available by the United States Centers for Disease Control and Prevention (U.S. CDC).

For additional details, please visit SigmaAldrich.com/ncov.

Other Notes

The product includes 1 tube of a 20X primer and probe pool for quantification of a SARS-CoV-2 gene. Assay included is N2.

The forward primer, reverse primer, and probe are at final concentrations of 10, 10, and 2.5 μM, respectively.

N2 Assay:

Forward Primer TTACAAACATTGGCCGCAAA
Reverse Primer GCGCGACATTCCGAAGAA
Dual-Labeled Probe ACAATTTGCCCCCAGCGCTTCAG

Legal Information

FAM is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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分析证书(COA)

Lot/Batch Number

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