Merck
CN

CE0050

Sigma-Aldrich

CelLytic MEM 蛋白提取试剂盒

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别名:
Membrane protein extraction kit
NACRES:
NA.56

技术

protein extraction: suitable

质量水平

运输

wet ice

储存温度

−20°C

一般描述

CelLytic MEM蛋白提取试剂盒提供了一种分离细胞中疏水性筏状微结构域相关蛋白的快速、方便的方法。该方法基于相分离,不需要分离细胞膜。分离的蛋白质可用于进一步的实验,例如 SDS-PAGE、蛋白质印迹、斑点印迹和免疫沉淀。 该试剂盒已在 HeLa、HEK-293、NIH 3T3、COS 和 CHO 及其它细胞系上进行了测试。
膜蛋白占据了生物体基因组大约20-30%的成分,充当着胞内的看门人、调节器和感应器的角色。它们具有多样的细胞功能,例如将细胞与外部的毒素隔离开来,作为胞内信号级联的起始点,以及维持临界离子浓度。

应用

CelLytic MEM 蛋白质提取试剂盒已被用于以肝细胞测定脂肪从头 合成和提取用于蛋白质印迹的膜蛋白。

适用性

提供足够进行80次检测的试剂。

法律信息

CelLytic is a trademark of Sigma-Aldrich Co. LLC

仅试剂盒组分

产品编号
说明

  • Lysis and Separation Buffer 50 mL

  • Wash Buffer for CelLytic MEM 50 mL

  • Sodium Chloride, 4M Solution 1.5 mL

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 1 mL化学品安全说明书

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Eye Irrit. 2 - Skin Irrit. 2

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

188.6 °F - closed cup

闪点(°C)

87 °C - closed cup

法规信息

常规特殊物品

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