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Merck
CN

CGD1

Sigma-Aldrich

细胞生长测定试剂盒，基于MTT法

别名:

Mitochondrial activity assay

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关于此项目

UNSPSC代码:
12352207
NACRES:
NA.75

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包装

pkg of 1 kit

质量水平

200

技术

UV/Vis spectroscopy: suitable
protein quantification: suitable

Amax

570 nm

应用

cell analysis
detection

检测方法

colorimetric

运输

dry ice

储存温度

−20°C

相关类别

一般描述

细胞生长测定试剂盒（基于MTT）设计用于以分光光度法测量细胞生长，作为活细胞中线粒体活性的函数。MTT(3-(4,5 -二甲基噻唑-2)- 2,5 -二苯基溴化四唑)系统是一种简单、准确、可重复性的方法，可通过线粒体脱氢酶活性测量活细胞活性。

应用

细胞生长测定试剂盒（基于MTT）已用于：
  • 测定成骨前细胞的细胞活力
  • 正常人前列腺间质和上皮细胞的MTT(3-(4,5 -二甲基噻唑-2)- 2,5 -二苯溴化四唑)检测
  • 测定原代宫颈上皮细胞的细胞活力

包装

每个试剂盒包括5瓶试剂，每瓶含1 mL 5 mg/ml MTT的RPMI溶液（无酚红）和50 mL MTT溶剂（0.1N盐酸的无水异丙醇溶液）。 优化后的各组分体积大小尤其适合多孔板细胞生长。 MTT溶液按总培养体积的10%添加，因此每个试剂盒的测试次数取决于培养容量和/或体积大小。

警示用语：

Danger

危险分类

Eye Irrit. 2 - Flam. Liq. 2 - Muta. 2 - STOT SE 3

靶器官

Central nervous system

储存分类代码

3 - Flammable liquids

闪点(°F)

53.6 °F - closed cup

闪点(°C)

12 °C - closed cup

法规信息

新产品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000462754
SLCP8351
SLCM0387
SLCK0987
SLCF3922

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Can we use media with phenol red with Product CGD1, Cell Growth Determination Kit?

    You most likely would not be able to use a medium containing phenol red with this kit. We have found that the phenol red can contribute to higher background, and therefore lower sensitivity.

  4. What is the difference between Product No. CGD1, Cell Growth Determination Kit and Product No.TOX1, In Vitro Toxicology Assay Kit?

    Product No. CGD1 and TOX1 are both MTT based assays. The major difference between Product No. CGD1 and TOX1 is that TOX1 contains 10 % Triton X-100 in the solubilization solution for better lysis of the cells and solubilization of the MTT.  If there is complete cell lysis, the results obtained with both kits would be the same.  The MTT in this kit is a solution, whereas the TOX1 assay kit contains the MTT as a powder that is solubilized before use.

  5. What are the components of the Product No. CGD1, Cell Growth Determination Kit and Product No.TOX1, In Vitro Toxicology Assay Kit?

    CGD1 contains 5 vials of 5 mg/vial of MTT and 50 ml of MTT solvent (solubilization solution) and TOX1 contains 5 vials of 15 mg/vial of MTT and 125 ml of solubilization solution.

  6. Is 10% fetal bovine serum (FBS) in the culture medium compatible with Product CGD1, Cell Growth Determination Kit?

    High protein levels may form a precipitate when the MTT solubilization solution is added. Samples with protein concentrations equivalent to 10% FBS or 4 mg/mL protein seem acceptable.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. Is the MTT assay quantitative?

    The MTT asay is a method to assess cell viability.  This assay is a semiquantitative assay. The assay is used to compare the viability changes in treated cells to untreated cells. The absorbance is indicative of the cell number. The higher the absorbance, the greater the number of viable cells present. Most researchers compare the absorbance of the two samples as a ratio (ABS treated cells/ABS untreated cells) to get a fold increase/decrease in cell number.   

  10. What is the difference between product CGD1, Cell Growth Determination Kit and product TOX1, In Vitro Toxicology Assay Kit?

    Product CGD1 and TOX1 are both MTT based assays. The major difference between product CGD1 and TOX1 is that TOX1 contains 10 % Triton X-100 in the solubilization solution for better lysis of the cells and solubilization of the MTT.  If there is complete cell lysis, the results obtained with both kits would be the same.  The MTT in this kit is a solution, whereas the TOX1 assay kit contains the MTT as a powder that is solubilized before use.

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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