Quality Level
usage
sufficient for 100 tests
shelf life
Kit is stable for 24 months when unopened.
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
General description
细胞谷胱甘肽过氧化物酶活性检测试剂盒用于对组织提取物中的谷胱甘肽过氧化物酶进行定量检测。本试剂盒基于谷胱甘肽（GSH）氧化后可生成氧化型谷胱甘肽（GSSG）。利用谷胱甘肽过氧化物酶（GPx）将GSH氧化成GSSG，生成的GSSG在谷胱甘肽还原酶（GR）的催化下再次生成GSH以及NADP+NAPDH氧化为NADP+的过程伴随340 nm处吸收光的减少。此处吸收值减少与样本中谷胱甘肽过氧化酶活性呈正相关。
Application
适用于测定组织提取物中的谷胱甘肽过氧化物酶
Biochem/physiol Actions
谷胱甘肽过氧化物酶细胞活性测定试剂盒用于测定组织提取物中的谷胱甘肽过氧化物酶。该测定基于将谷胱甘肽（GSH）氧化为氧化型谷胱甘肽（GSSG）。这一反应由GPx催化，并用谷胱甘肽还原酶和NADPH将GSSG再循环回GSH。由于该酶是偶联反应的限速因子，因此在NADPH氧化成NADP的过程中，可通过测定340nm处NADPH吸光度的降低，表明谷胱甘肽过氧化物酶的活性。
Other Notes
本试剂盒通过了兔网织红细胞裂解液和谷胱甘肽过氧化物酶的测试。
仅试剂盒组分
产品编号
说明
- tert-Butyl hydroperoxide 1 mL
signalword
Danger
Hazard Classifications
Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Org. Perox. F - Skin Corr. 1C - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
存储类别
5.2 - Organic peroxides and self-reacting hazardous materials
法规信息
常规特殊物品
危险化学品
此项目有
International Committee for Standardization in Haematology: recommended methods for red-cell enzyme analysis.
E Beutler et al.
British journal of haematology, 35(2), 331-340 (1977-02-01)
Ian M Scott et al.
Pesticide biochemistry and physiology, 141, 9-17 (2017-09-16)
Plant elicitors can be biological or chemical-derived stimulators of jasmonic acid (JA) or salicylic acid (SA) pathways shown to prime the defenses in many crops. Examples of chemical elicitors of the JA and SA pathways include methyl-jasmonate and 1,2,3-benzothiadiazole-7-carbothioate (BTH
Diversity of glutathione peroxidases.
F Ursini et al.
Methods in enzymology, 252, 38-53 (1995-01-01)
Matthias L Jauslin et al.
Human molecular genetics, 11(24), 3055-3063 (2002-11-06)
Friedreich Ataxia (FRDA), the most prevalent of the inherited ataxias, is a multi-systemic disease with loss of sensory neurons and life-threatening hypertrophic cardiomyopathy as its most severe manifestations. Reduced levels of the mitochondrial protein frataxin lead to cell-damaging oxidative stress
V R Arruda et al.
Neoplasma, 43(2), 99-102 (1996-01-01)
Red cell antioxidant enzymes have been recently studied in malignant lymphomas and the results are controversial. Hairy cell leukemia is a rare chronic lymphoproliferative disorder originating probably in a pluripotent stem cell. In the present study, glutathione peroxidase (Gpx), reduced
商品
Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.
氧化应激，在一部分上，是由多种细胞过程产生的活性氧介导，并由诸如酶清除剂或抗氧化剂调节剂等细胞抗氧化剂机制所控制的。自由基，如活性氧，会通过细胞引起细胞损伤。
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| CGP1-1KT | 04061833531761 |
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持