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CGP1

Sigma-Aldrich

细胞谷胱甘肽过氧化物酶活性检测试剂盒

Sufficient for 100 colorimetric tests

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别名:
c-GPx, 细胞谷胱甘肽过氧化物酶
NACRES:
NA.32

质量水平

200

用途

sufficient for 100 tests

保质期

Kit is stable for 24 months when unopened.

检测方法

colorimetric

运输

wet ice

储存温度

−20°C

相关类别

一般描述

细胞谷胱甘肽过氧化物酶活性检测试剂盒用于对组织提取物中的谷胱甘肽过氧化物酶进行定量检测。本试剂盒基于谷胱甘肽（GSH）氧化后可生成氧化型谷胱甘肽（GSSG）。利用谷胱甘肽过氧化物酶（GPx）将GSH氧化成GSSG，生成的GSSG在谷胱甘肽还原酶（GR）的催化下再次生成GSH以及NADP+NAPDH氧化为NADP+的过程伴随340 nm处吸收光的减少。此处吸收值减少与样本中谷胱甘肽过氧化酶活性呈正相关。

适用性

适用于测定组织提取物中的谷胱甘肽过氧化物酶

原理

谷胱甘肽过氧化物酶细胞活性测定试剂盒用于测定组织提取物中的谷胱甘肽过氧化物酶。该测定基于将谷胱甘肽（GSH）氧化为氧化型谷胱甘肽（GSSG）。这一反应由GPx催化，并用谷胱甘肽还原酶和NADPH将GSSG再循环回GSH。由于该酶是偶联反应的限速因子，因此在NADPH氧化成NADP的过程中，可通过测定340nm处NADPH吸光度的降低，表明谷胱甘肽过氧化物酶的活性。

其他说明

本试剂盒通过了兔网织红细胞裂解液和谷胱甘肽过氧化物酶的测试。

仅试剂盒组分

产品编号
说明
  • tert-Butyl hydroperoxide 1 mL

相关产品

产品编号
说明
价格

警示用语：

Danger

危险分类

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Org. Perox. F - Skin Corr. 1C - Skin Sens. 1 - STOT SE 3

靶器官

Respiratory system

储存分类代码

5.2 - Organic peroxides and self-reacting hazardous materials

WGK

WGK 3

法规信息

常规特殊物品
危险化学品

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示例

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索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Can Product CGP1, Glutathione Peroxidase Cellular Activity Assay Kit, be run in a 96 well plate?

    Yes, but you would need to have a plate reader that has a kinetic function as this is a kinetic assay.

  4. How should I prepare my sample for analysis with Product CGP1, Glutathione Peroxidase Cellular Activity Assay Kit?

    The cells must be lysed before assaying with this kit.  The best way to perform lysis is to incubate the cells with hypotonic buffer (at least 2 volumes and better with 3-4 volumes, depending on the expected level of peroxidase in these cells). Let them swell and homogenize with a Dounce homogenizer. Centrifuge the homogenate at 10,000 × g and use the supernatant for the assay.  If a detergent is used for lysis, only use a peroxide-free detergent and follow the instructions in the Bulletin: "Nonionic detergents such as TWEEN and TRITON X-100 that contain high levels of endogenous peroxides will raise the apparent activity. If these detergents are vital to the extraction of the proteins of interest, a low peroxide detergent should be used such as Product Codes X-100-PC (TRITON X-100), P 6585 (TWEEN 20), or P 8192 (TWEEN 80)".

  5. Can Product CGP1, Glutathione Peroxidase Cellular Activity Assay Kit, be used with tissue samples?

    We have not tested the CGP1 assay with tissue samples. However, we suggest that the customer perform a small-scale pilot experiment using our CelLytic-MT lysis buffer (Product No. C3228). The buffer enables efficient extraction of tissue proteins. It does not contain any of the interfering agents that are listed on the kit bulletin.

  6. What type of readings do I get with Product CGP1, Glutathione Peroxidase Cellular Activity Assay Kit?

    There is a decrease in absorbance during the assay. The OD values decrease with the increase in reaction time. The slope of the reduction in OD (delta OD per minute) is the value used to determine the enzyme units.

  7. What dilution should I use for my sample when using Product CGP1, Glutathione Peroxidase Cellular Activity Assay Kit?

    In general, when human erythrocytes were assayed for the enzyme, the sample was diluted 10-fold (15 mg peroxidase per mL) and a 20 μl sample gave a decrease of 0.032 OD340 per minute. Too much enzyme will underestimate the apparent activity of the sample. When rabbit reticulocytes were assayed at a 10-fold dilution (10 mg peroxidase per mL), 20 μl gave a decrease of 0.065 OD340 per minute.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

V R Arruda et al.
Neoplasma, 43(2), 99-102 (1996-01-01)
Red cell antioxidant enzymes have been recently studied in malignant lymphomas and the results are controversial. Hairy cell leukemia is a rare chronic lymphoproliferative disorder originating probably in a pluripotent stem cell. In the present study, glutathione peroxidase (Gpx), reduced
Diversity of glutathione peroxidases.
F Ursini et al.
Methods in enzymology, 252, 38-53 (1995-01-01)
Ian M Scott et al.
Pesticide biochemistry and physiology, 141, 9-17 (2017-09-16)
Plant elicitors can be biological or chemical-derived stimulators of jasmonic acid (JA) or salicylic acid (SA) pathways shown to prime the defenses in many crops. Examples of chemical elicitors of the JA and SA pathways include methyl-jasmonate and 1,2,3-benzothiadiazole-7-carbothioate (BTH
Matthias L Jauslin et al.
Human molecular genetics, 11(24), 3055-3063 (2002-11-06)
Friedreich Ataxia (FRDA), the most prevalent of the inherited ataxias, is a multi-systemic disease with loss of sensory neurons and life-threatening hypertrophic cardiomyopathy as its most severe manifestations. Reduced levels of the mitochondrial protein frataxin lead to cell-damaging oxidative stress
I Mavelli et al.
European journal of biochemistry, 139(1), 13-18 (1984-02-15)
Red blood cells of favism patients with acute hemolytic crisis have markedly more superoxide dismutase (superoxide:superoxide oxidoreductase, EC 1.15.1.1) and less glutathione peroxidase (glutathione:hydrogenperoxide oxidoreductase, EC 1.11.1.9) than either normal controls, glucose-6-phosphate dehydrogenase-deficient subjects or favism patients outside hemolytic crisis.
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