产品名称
Imprint® Ultra染色质免疫沉淀试剂盒，不含对照品, ChIP kit for maximum sensitivity, compatible with Next-Gen sequencing
Quality Level
相关类别
Application
- 适用于下游应用
- 从单个靶标表征到全基因组分析的技术
- 信号转导途径的表征
- ChIp-chIP和ChIP-seq数据的验证
Features and Benefits
- 成功采用与低丰度、中等和高表达转录因子以及组蛋白修饰相关的 CHiP′ed DNA。
- 更大的容量 - 可用于 2-10 × 106 的各种细胞数目范围
- 最高的灵敏度 - 能够检测低至 2 × 106个细胞的低丰度转录因子
- 采用 DNA 封闭的 "Staph-Seq" 进行IP（免疫沉淀），最大限度减少下游 ChIP-Seq 应用中的葡萄球菌A DNA 污染。
General description
Imprint Ultra Chromatin IP 试剂盒是 Sigma′第二代染色质免疫沉淀（ChIP） 试剂盒，旨在实现最高灵敏度和最佳下一代测序结果。它提供了一套完整的染色质免疫沉淀解决方案，包括用于 DNA 纯化的色谱柱和试剂。该试剂盒让研究人员可以探索低丰度转录因子（TF） 的全基因组结合位点，以及新的组蛋白修饰。它针对来自 106 个细胞（最多约 50 μg DNA）的染色质的 ChIP 反应进行了优化，也可以扩大规模（或合并多个制剂）以适用于108 个细胞，用于 ChIP-chip 和 ChIP-Seq 应用中的全基因组结合研究。
Legal Information
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Ox. Liq. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
target_organs
Central nervous system
存储类别
5.1A - Strongly oxidizing hazardous materials
法规信息
监管及禁止进口产品
此项目有
Comparison of sample preparation methods for ChIP-chip assays.
O'Geen, H., et al.
Biotechniques, 41, 557-557 (2006)
Chromatin isolation by RNA purification (ChIRP).
Chu, C., et al.
Journal of Visualized Experiments (2012)
Philippe Collas
Molecular biotechnology, 45(1), 87-100 (2010-01-16)
The biological significance of interactions of nuclear proteins with DNA in the context of gene expression, cell differentiation, or disease has immensely been enhanced by the advent of chromatin immunoprecipitation (ChIP). ChIP is a technique whereby a protein of interest
Genomic maps of long noncoding RNA occupancy reveal principles of RNA-chromatin interactions.
Chu, C., et al.
Molecular Cell (2011)
M H Kuo et al.
Methods (San Diego, Calif.), 19(3), 425-433 (1999-12-02)
Chromatin structure plays important roles in regulating many DNA-templated processes, such as transcription, replication, and recombination. Considerable progress has recently been made in the identification of large, multisubunit complexes dedicated to these nuclear processes, all of which occur on nucleosomal
实验方案
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
相关内容
蛋白质是细胞内部的主要功能分子，负责细胞功能和生存的多种生物学活动。值得关注的是，许多蛋白质也能与DNA发生相互作用。
Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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