A549 Cells SMAD4 -/-

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

Media Renewal changes two to three times per week

Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping O-ring and cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.

Subculture Ratio: approx. 1:3 to 1:8

The base medium for this cell line is F12 Ham Medium, Cat. No. N4888. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10% and L-glutamine, Cat. No. G7513, at a final concentration of 2mM.

The cell freezing medium is DMSO 1X (Cat. No. C6164)

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

These A549 cells are adherent, with a doubling time of approx. 22 hours.

Zinc finger nuclease (ZFN) knock out on chromosome 18q21.1

This product corresponds to ATCC Cat. No. CCL-185