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安全信息

CMC0014

Sigma-Aldrich

BL21（DE3）化学感受态细胞

for protein expression

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NACRES:
NA.85

生物来源

Escherichia coli

等级

for molecular biology

生长模式

adherent or suspension

形态学

rod shaped

技术

microbiological culture: suitable

细胞转化

competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg

运输

dry ice

储存温度

−70°C

相关类别

一般描述

BL21（DE3）化学感受态菌株含有与IPTG诱导型启动子连接的噬菌体T7 RNA聚合酶基因，可用于含有T7启动子的任何表达质粒。

基因型

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

应用

该 大肠杆菌 BL21（DE3）菌株已用于：以pET19b作为表达载体，表达tau 2N4R同种型（aa1–441）和截短的tau（aa1–421）。
  • BL21(DE3)化学感受态细胞已被用于protegrin-1 (PG1) 肽的转化和表达。
  • 在 pHis2载体中表达6His-TEVsite-Hs ARF1 (17-181)-Q71L。

    • 特点和优势

    • 转化效率> 1×107 CFU /µg
    • 实惠的价格
    • 可为日常蛋白表达工作提供卓越价值。

    组分

    • BL21（DE3）化学感受态细胞
    • pUC19转化对照DNA
    • 表达恢复培养基

    相关产品

    产品编号
    说明
    价格

    L3022

    LB 肉汤（Lennox）, Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.

    L2897

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    L5542

    LB Agar Plates, pre-poured LB agar plates for E. coli

    B11001

    EnPresso® B, Growth system for expressing protein in bacteria

    H9950

    Hanahan′s Broth (SOB Medium), Liquid microbial growth medium

    CMC0015

    BL21（DE3）pLysE化学感受态细胞, for protein expression

    CMC0017

    过表达 ™ C41 (DE3) 化学感受态细胞, for the highest protein expression

    CMC0023

    CONTROLLER SIG10化学感受态细胞, for controlled protein expression

    CMC0024

    CONTROLLER BL21(DE3)化学感受态细胞, for controlled protein expression

    CMR0001

    Recovery Medium for Expression, for use with competent cells

    储存分类代码

    10 - Combustible liquids

    WGK

    WGK 3

    法规信息

    动植物来源培养基
    常规特殊物品

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    Yasaman Damestani et al.
    Lasers in surgery and medicine, 48(8), 782-789 (2016-10-19)
    The development and feasibility of a novel nanocrystalline yttria-stabilized-zirconia (nc-YSZ) cranial implant has been recently established. The purpose of what we now call "window to the brain (WttB)" implant (or platform), is to improve patient care by providing a technique
    David B Sauer et al.
    eLife, 9 (2020-09-02)
    Citrate, α-ketoglutarate and succinate are TCA cycle intermediates that also play essential roles in metabolic signaling and cellular regulation. These di- and tricarboxylates are imported into the cell by the divalent anion sodium symporter (DASS) family of plasma membrane transporters
    A validated antibody panel for the characterization of tau post-translational modifications.
    Ebru E, et al.
    Mol. Neurodegener., 12(1), 87-87 (2017)
    Romana Koszagova et al.
    Microbial cell factories, 17(1), 139-139 (2018-09-05)
    Physiological aggregation of a recombinant enzyme into enzymatically active inclusion bodies could be an excellent strategy to obtain immobilized enzymes for industrial biotransformation processes. However, it is not convenient to recycle "gelatinous masses" of protein inclusion bodies from one reaction
    Gints Kalnins et al.
    Nature communications, 11(1), 388-388 (2020-01-22)
    Bacterial microcompartments (BMCs) are prokaryotic organelles consisting of a protein shell and an encapsulated enzymatic core. BMCs are involved in several biochemical processes, such as choline, glycerol and ethanolamine degradation and carbon fixation. Since non-native enzymes can also be encapsulated
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    BL21 Chemically Competent Cells

    BL21 Chemically Competent Cells are provided in aliquots of 80 μL sufficient for two transformation reactions of 40 μL each. Transformation is performed by heat shock at 42 °C, followed by incubation on ice.

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