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质量水平
形式
liquid
包装
vial of 50 μL
浓度
20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
应用
CRISPR
运输
dry ice
储存温度
−20°C
一般描述
OCT4 is the key transcription factor (TF) that maintains pluripotency of embryonic stem cells and plays a critical role in reinstating cellular pluripotency. The expression of OCT4 is stringently silenced in differentiated cells. Activation of silenced OCT4 gene has become a hallmark event during epigenetic reprogramming into iPSCs. The positive control OCT4 gRNA design has been tested with the dCas9-p300 plasmid in HEK293 cells and shown to increase OCT4 mRNA transcript levels over three-fold compared to a negative control.
应用
Functional Genomics/Target Validation
- Epigenetic Modification
- Transcriptional Activation
特点和优势
Serves as an experimental control for the CRISPR dCas9-p300 activation system. A positive experiment will result in an increased level of mRNA via qPCR or TaqMan PCR assay.
组分
1 vial containing 1ug of U6-gRNA plasmid expressing a guide sequence to human OCT4 (Octamer-binding transcription factor-4 or POU5F1).
联系
To order U6-gRNA plasmids, please visit sigma.com/crisprs
Click here for more information on dcas9-p300 Gene Activator Expression Plasmid.
Click here for more information on dcas9-p300 Gene Activator Expression Plasmid.
其他说明
Must be used with dCas9-p300 or other dCas9 activator/repressor plasmids.
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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