应用
该试剂盒包含有效检测BACE1活性所需的全部试剂。其中包含筛选潜在BACE1抑制剂的酶。该检测基于荧光共振能量转移 (FRET) 技术，即在BACE1切割底物后观测增强的荧光信号。
生化/生理作用
作为一种跨膜蛋白酶，BACE1负责切割淀粉样前体蛋白 (APP) 的β位点来产生β淀粉样肽 (Aβ)。大脑中Aβ积累是阿尔茨海默氏症的主要病因。BACE1是抑制剂药物研发的靶点。
仅试剂盒组分
产品编号
说明
- Fluorescent Assay Buffer 50 mL
- Stop Solution 15 mL
- Substrate (MOCA-SEV-NL-DAEFR-DNP-RR) 500 μL
- Assay Standard 140 μL
- BACE1 (β−Secretase) 300 units 100 μL
储存分类代码
10 - Combustible liquids
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Vijay K Nuthakki et al.
Bioorganic chemistry, 90, 103062-103062 (2019-06-21)
Alkaloids have always been a great source of cholinesterase inhibitors. Numerous studies have shown that inhibiting acetylcholinesterase as well as butyrylcholinetserase is advantageous, and have better chances of success in preclinical/ clinical settings. With the objective to discover dual cholinesterase
Changxing Qi et al.
Fitoterapia, 130, 134-139 (2018-08-31)
Terrusnolides A-D (1-4), four butenolides were isolated from an endophytic Aspergillus from Tripterygium wilfordii. The structures of 1-4 were established by comprehensive spectroscopic analyses and electronic circular dichroism (ECD) calculation. It is interesting that 1 was a butenolide derived by
Raúl Horacio Camarillo-López et al.
Molecules (Basel, Switzerland), 25(21) (2020-11-04)
Alzheimer's disease (AD) is a neurodegenerative disease with no cure nowadays; there is no treatment either to prevent or to stop its progression. In vitro studies suggested that tert-butyl-(4-hydroxy-3-((3-(2-methylpiperidin-yl)propyl)carbamoyl)phenyl) carbamate named the M4 compound can act as both β-secretase and
Lucas J Gutierrez et al.
Journal of biomolecular structure & dynamics, 35(2), 413-426 (2016-01-28)
We report here two new small-size peptides acting as modulators of the β-site APP cleaving enzyme 1 (BACE1) exosite. Ac-YPYFDPL-NH2 and Ac-YPYDIPL-NH2 displayed a moderate but significant inhibitory effect on BACE1. These peptides were obtained from a molecular modeling study.
L Hong et al.
Biochemical Society transactions, 30(4), 530-534 (2002-08-28)
As beta-secretase, memapsin 2 cleaves amyloid-beta precursor protein, which leads ultimately to the onset of Alzheimer's disease. As such, memapsin 2 is an excellent target of inhibitor drugs for the treatment of this disease. Here we describe the tools for
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